Glicerol crudo (1,2, 3 propanotriol) de Jatropha curcas L. Su efecto en la dinámica fermentativa y ecología del rumen

Contenido principal del artículo

Juana L. Galindo
J.A. Sotolongo
Daiky Valenciaga
Magaly Herrera
Á. Delgado

Resumen

To evaluate the effect of crude glycerol from Jatropha curcas L. on the fermentation dynamics and rumen ecology, an in vitro experiment was conducted. The treatments were: 1) star grass + concentrate without glycerol (control), 2) star grass + concentrate with 3 % glycerol and 3) star grass + concentrate with 6 % glycerol. Sampling was carried out at 0 hours (before incubation) and at 3 and 6 hours after the start of incubation. The populations of total viable bacteria, cellulolytic, proteolytic and cellulolytic fungi were determined according to a completely random design in factorial arrangement. Nonparametric analysis of variance was performed. The populations of total viable bacteria, cellulolytic bacteria, and cellulolytic fungi were higher with 3 % glycerol, and there was no effect on the population of proteolytic bacteria. There were changes in the concentration of total short-chain fatty acids, their molar proportions and the acetic/propionic acid ratio. The estimated glucose release was 10788.67, 11200.33 and 12110.00 g. The methane produced was 751.56, 708.04 and 726.67 g and the microbial biomass was 4126.2, 4347.7 and 5621.5 g for the control (without glycerol), 3 and 6 %, respectively. It is concluded that glycerol modifies microbial populations and fermentative processes in the rumen. These studies are the first to use glycerol from J. curcas in the rumen.

Detalles del artículo

Cómo citar
Galindo, J. L., Sotolongo, J., Valenciaga, D., Herrera, M., & Delgado, Á. (2025). Glicerol crudo (1,2, 3 propanotriol) de Jatropha curcas L. Su efecto en la dinámica fermentativa y ecología del rumen. Cuban Journal of Agricultural Science, 59, https://cu-id.com/1996/v59e11. Recuperado a partir de https://www.cjascience.com/index.php/CJAS/article/view/1187
Sección
Ciencia Animal

Citas

Aita-Portillo, C.R. & Elizondo-Salazar, J.A. 2023. Efecto del uso de una mezcla de compuestos gluconeogénicos en vacas lecheras en transición. Agronomía Costarricense, 47(2): 11-120, ISSN: 2215-2202. https://dx.doi.org/10.15517/rac.v47i2.56136.

AOAC. 2016. Official methods of analysis of AOAC International. 20. ed. ed., Rockville MD: AOAC International., Latimer, George W. Jr., ISBN: 9780935584875, Available at: http://www.worldcat.org/title/official-meth-ods-of-analysis-of-aoac.international/oclc/981578728?re-ferer=null&ht=edition, [Consulted: April 05, 2018].

Awogbemi, O. & Desai, D.A. 2025. Progress in the conversion of biodiesel-derived crude glycerol into biofuels and other bioproducts. Bioresource Technology Reports, 30(1): 102106, ISSN: 2589-014X. https://doi.org/10.1016/j.bi-teb.2025.102106.

Bonis, R., Valenciaga, D., García López, R., Sotolongo, J.A. & Galindo, J.L. 2024. Effect of crude glycerol from Jatropha curcas L. oil on the production and quality of cattle milk. Cuban Journal of Agricultural Science, 58: e21, ISSN: 2079-3480. https://cu-id.com/1996/ v58e21, https://www.cjascience.com/index.php/CJAS/ar-ticle/view/1156.

Caldwell, D.R. & Bryant, M.P. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied Microbiology, 14(5): 794-801, ISSN: 2717-5936. https://doi.org/10.1128/am.14.5.794-801.

Chanjula, P., Pongprayoon, S., Kongpan, S. & Cherdthong, A. 2016. Effects of crude glycerin from waste vegetable oil supplementation on feed intake, ruminal fermentation characteristics, and nitrogen utilization of goats. Tropical Animal Health and Production, 48(5): 995-1004 ISSN: 1573-7438. https://doi.org/10.1007/s11250-016-1047-0.

Clariget, J.M., Pérez-Clariget R., Álvarez-Oxiley, A., Bentancur, O. & Bruni, M.Á. 2016. Suplementación con glicerina cruda y afrechillo de arroz entero a vacas de carne pastoreando campo natural. Agrociencia Uruguay, 20(2): 121-131, ISSN: 2301-1548. https://agrocienciauru-guay.uy/indeex.php/agrociencia/article/view/212.

Conover, W.J. 1999. Practical Nonparametric Statistics. Third Edition, John Wiley & Sons, New York. 608 pp. ISBN 9780471160687/0471160687. Correa, C. & Moreno, L. 2019. Evaluación de la producción de leche, nitrógeno ureico en sangre y algunos componentes de la leche en vacas Holstein suplementadas con glicerol y palmiste en la dieta. Revista Colombiana de Zootecnia, 5(10): 38-47, ISSN: 2462-8050. http://www.anzoo.org/publica-ciones/index.php/anzoo/article/view/95/91.

Cottyn, B.G. & Boucque, C.V. 1968. Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry, 16(1): 105-107, ISSN: 1520-5118. https://doi.org/10.1021/jf60155a002.

Delgado, A., Bruni, M.A., Galindo, J.L., Marchelli, J.P., Rodríguez, D. & Chilibroste, P. 2016. Efectos del glicerol al inicio de la lactancia en la producción y calidad de la leche de vacas Holando en pastoreo. Avances en Investigación Agropecuaria, 20(2): 5-18, ISSN: 2683-1716. https://www.redalyc.org/jour-nal/837/83754343002/83754343002.pdf.

Delgado, A., Bruni, M.A., Galindo, J.L., Marchelli, J.P., Rodríguez, D. & Chilibroste, P. 2018. Efecto de la sustitución de maíz por glicerol crudo sobre el consumo de materia seca, en vacas Holando en pastoreo. Pastos y Forrajes, 41(2): 131-137, ISSN: 2078-8452. http://scielo.sld.cu/scielo.php?script=sci_art-tex&pid=S0864-03942018000200007&Ing=es&nrm=iso.

Elias, A. 1971. The rumen bacteria of animals fed on a high- molasses urea diet. Thesis PhD. Aberdeen.

Filho, R.S.F., Rebelo, L.R., Zanchetin, M., Silva, A.S., de Paula, N.F., Zervoudakis, J.T., da Silva Cabral, L. & Galati, R.L. 2024. Parcial replacement of corn grain with levels of crude glycerin on feed intake, digestibility, ruminal fermentation, nitrogen utilization, and performance of feedlot lambs. Tropical Animal Health and Production, 56(9): 401, ISSN: 1573-7438. https://doi.org/10.1007/s11250-024-04245-y.

Fraga, D. da Rosa, Ulsenheimer, B.C., Pereira, E.A., da Silva, J.A.G., Baroni, J.I., Pereira, S.N., de Oliveira, L., Huttra, A.P. & Viégas, J. 2024. Milk composition and productivity of Holstein cows in Ryegrass grazing and crude glycerin in the diet. Revista de Gestão Social e Ambiental, 18(2): e03635, ISSN: 1981-982X. https://doi.org/10.24857/rgsa.v18n2-077.

Gaillard, C., Sorensen, M., Vestergaard, M.R., Weisbjerg, M.K., Larsen, H., Martinussen, U. & Sehested, J. 2018. Effect of substituting barley with glycerol as energy feed on feed intake, milk production and milk quality in dairy cows in mid or late lactation. Livestock Science, 209: 25-31, ISSN: 1878-0490. https://doi.org/10.1016/j.livs- ci.2018.01.006.

Galindo, J. 1988. Efecto de la zeolita en la población de bacterias celulolíticas y su actividad en vacas que consumen ensilaje. Tesis en opción al grado científico de Dr.C. Veterinarias. Instituto de Ciencia Animal, Cuba.

Galindo, J., Bonis, R., Valenciaga, D., Sotolongo, J.A., Soca, M., Delgado, A., García, R., Herrera, M. & Suárez, J. 2025. Evaluación del glicerol (1,2, 3 propanotriol), procedente del aceite de Jatropha curcas: su efecto en la ecología ruminal y producción de leche vacuna. Informe Final de Proyecto de investigación PN131LH001.48. Evaluación del glicerol (1,2,3 propanotriol), procedente de Jatropha curcas en la fisiología ruminal y comportamiento productivo en vacunos de leche y carne. Instituto de Ciencia Animal. Mayabeque, Cuba.

Gómez, L. & Campos, R. 2016. Control del balance energético negativo y comportamiento productivo y metabólico en vacas doble propósito bajo suplementación energética. Revista de Investigación Agraria y Ambiental, 7(1): 147-156, ISSN: 2145-6453. https://doi.org/10.1016/10.22490/21456453.1545.

Granja-Salcedo Y.T., de Souza V.C., Dias A.V., Gomez- Insuasti A.S., Messana J.D. & Berchielli T.T. 2017. Diet containing glycerine and soybean oil can reduce ruminal biohydrogenation in Nellore steers. Animal Feed Science and Technology, 225: 195-204, ISSN: 1873-2216. https://doi.org/10.1016/j.anifeedsci.2017.01.021.

Hejna, A., Kosmela, P., Formela, K., Piszczyk, Ł. & Haponiuk, J.T. 2016. Potential applications of crude glycerol in polymer technology-Current state and perspectives. Renewable and Sustainable Energy Reviews, 66(C): 449-475, ISSN: 1879-0690. http://dx.doi.org/10.1016/j.rser.2016.08.020.

Herrera, M. Bustillo, C.W. & Torres, V. 2015. Metodología para el análisis estadístico de diferentes de tipos de variables que se miden en las investigaciones que utilizan diseños experimentales relacionados con los modelos de análisis de varianza paramétrico y no paramétrico. ISBN 978-959-7171-57-7. Editorial EDICA, Instituto de Ciencia Animal, Cuba.

Hidalgo-Hernández, U., Ortega-Cerrilla, M.E., Herrera-Haro, J.G., Ramírez-Mella, M. & Zetina-Córdoba, P. 2018. Glicerol una alternativa para la alimentación de rumiantes. Agro Productividad, 11(5): 124-129, ISSN: 2594-0252. https://doi.org/10.22004/ag.econ.352916.

Hungate, R.G. 1950. The anaerobic, mesophilic cellulolytic bacteria. Bacteriological Reviews, 14(1): 1-49, ISSN: 2691-9443. https://doi.org/10.1128/br.14.1.1-49.1950.

Balzarini, M., Di Rienzo, A., Cazanove, F., González, L., Tablada, M., Guzmán, W. & Robeldo, W. 2012. InfoStat paquete estadístico InfoStat versión 2012.

Joblin, K.N. 1981. Isolation, enumeration and maintenance of rumen anaerobic fungi in roll tubes. Applied and Environmental Microbiology, 42(6): 1119-1122, ISSN: 1098-5336. https://doi.org/10.1128/aem.42.6.1119-1122.1981.

Kansagara, Y.G., Savsani, H.H., Chavda, M.R., Chavda, J.A., Belim, S.Y., Makwana, K. & Kansagara, B.K. 2022. Rumen microbiota and nutrient metabolism: A Review. Bhartiya Krishi Anusandhan Patrika, 37(4): 320- 327, ISSN: 0976-4631. https://doi.org/10.18805/BKAP486.

Kruskal, W. & Wallis, W. 1952. Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association, 47(260): 583-621, ISSN: 1537-274X. https://doi.org/10.2307/2280779.

Ladeira, M.M., Carvalho, J.R.R., Chizzotti, M.L., Teixeira, P.D., Dias, J.C.O., Gionbelli, T.R.S., Rodrigues, A.C. & Oliveira, D.M. 2016. Effect of increasing levels of glycerin on growth rate, carcass traits and liver gluconeogenesis in young bulls. Animal Feed Science and Technology, 219: 241-248, ISSN: 1873-2216. http://dx.doi.org/10.1016/j.anifeedsci.2016.06.010.

Levene, H. 1960. Robust tests for the equality of variance. Contributions to Probability and Statistics. Stanford University Press.

Li, Y., Wang, H., Zhang, Y., Li, X., Jiang, X. & Ding, H. 2022. Effects of dietary supplementation with glycerol monolaurate (GML) or the combination of GML and tributyrin on growth performance and rumen microbiome of weaned lambs. Animals, 12(10): 1309, ISSN: 2076-2615. http://doi.org/10.3390/ani12101309.

Marchelli, J.P., Bruni, M.A. & Chilibroste, P. 2015. Efecto de la sustitución de grano de maíz por glicerol crudo sobre el consumo y patrón de fermentación. Archivos Latinoamericanos de Producción Animal, 23(5): 79-80, ISSN: 2075-8359. http://www.alpa.org.ve/ojs/in-dex.php/ojs_files/article/viewFile/2516/903.

Menke, K.H. & Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28: 7-55, ISSN: 0340-3165.

Ningaraju, C., Yatish, K. & Sakar, M. 2022. Simultaneous refining of biodiesel-derived crude glycerol and synthesis of value-added powdered catalysts for biodiesel production: a green chemistry approach for sustainable biodiesel industries. Journal of Cleaner Production, 363: 132448, ISSN: 0959-6526. https://doi.org/10.1016/j.jcle-pro.2022.132448.

Nivia-Osuna, A., Ramírez-Peña, A., Porras-Sánchez, C.J. & Marentes-Barrantes, D.L. 2020. Glicerol: Suplemento alimenticio y su respuesta en bovinos de leche. Agronomía Mesoamericana, 31(3): 821-833, ISSN: 2215-3608. http://doi.org/10.15517/am.v31i3.39259.

Piloto, R., Sotolongo, J.A., Díaz, Y. & Suárez, J. 2021. Extracción de aceite de origen vegetal. En: Biodiésel: producción y uso. Capítulo 2. Editor: Dr.C. Ramón Piloto Rodríguez, Dr.C. Jesús Suárez Hernández y M.Sc. José Angel Sotolongo Pérez. ISBN: 978-959-7138-48-8.

Shapiro, S. & Wilk, B. 1965. An analysis of variance test for normality (complete simples). Biometrika, 52(3-4): 591-611, ISSN: 1464-3510. https://doi.org/10.2307/2333709.

Smith, R.H. 1975. Nitrogen metabolism in the rumen and the composition and nutritive value of nitrogen compounds entering the duodenum. En: Digestion and metabolism in the Ruminant. W. McDonald & A.C.I. Warner (eds.) New England University. Publishing Unit. Armidale. Australia. p. 399

Sotolongo J.Á., Piloto R., Díaz A. & Hernández J. 2021. Producción de biodiesel. En Libro Biodiésel: producción y uso. Capítulo 4 Editor: Dr.C. Ramón Piloto Rodríguez, Dr.C. Jesús Suárez Hernández y M.Sc. José Ángel Sotolongo Pérez. ISBN 978-959-7138-48-8.

Stuart, R. 2016. BALANCE-RUMETANO: programa estadístico para el cálculo del balance estequiométrico de la fermentación ruminal. Mayabeque, Cuba: Instituto de Ciencia Animal.

Theodorou, M.K., Williams, B. A., Dhanoa, M.S., McAllan, A.B. & France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48(3): 185-197, ISSN: 2321-1628. https://doi.org/10.1016/0377-8401(94)90171-6.

Valencia-Echavarría, D.M., Granja-Salcedo, Y.T., Noriega- Marquez, J.G., Valderrama, L.A.G., Vargas, J.A.C. & Berchielli, T.T. 2024. Crude glycerol increase neutral detergent fiber degradability and modulates rumen fermentative dynamics of kikuyu grass in non-lactating Holstein cows raised in tropical conditions. Dairy, 5(3): 480-490, ISSN: 2624-862X. https://doi.org/10.3390/dai-ry5030037.

Van-Cleef, E.H.C.B., Sancanari, J.B.D., Silva, Z.F., D’Aurea, A.P., Favaro, V.R., van Cleef, F.O.S., Homem Júnior, A.C. &. Ezequiel. J.M.B. 2016. High concentrations of crude glycerin on ruminal parameters, microbial yield, and in vitro greenhouse gases production in dairy cows. Canadian Journal of Animal Science, 96(4): 461-465, ISSN: 1918-1825. https://doi.org/10.1139/cjas-2015-0170.

Vera, N. Suescun-Ospina, T., Gutierrez-Gomez, C., Olms- Salvo, V. & Aviala-Stagno, J. 2025. Effects of linseed and glycerol inclusion in concentrate ruminant diets on methane production using a Rusitec semicontinuous system. Chilean Journal of Agricultural Research, 85(3): 434-444, ISSN: 0718-5839. http://dx.doi.org/10.4067/s0718-58392025000300434.

Vesga, D.A., Granja-Salcedo, Y.T., Costa, R.V., Gomes, K.L., Carvalho Alves, Narvaez, H.J. & Berchielli, T.T. 2024. Changes in ruminal fermentation and rumen bacteria population in feedlot cattle during a high lipid diet adaptation. Animal Science Papers and Reports, 42(3): 255-270, ISSN: 230-8342. https://doi.org/10.2478/aspr-2023-0035.

Zacaroni, O.F., Lopes, L.M., Júnior, G.S.D., DeVries, T.J., Pereira, R.A., Donkin, S.S. & Pereira, M.N. 2022. Complete replacement of corn grain with crude glycerin for dairy cows. Livestock Science, 258: 104893, ISSN: 1878-0490. https://doi.org/10.1016/j.livsci.2022.104893.

Artículos similares

También puede {advancedSearchLink} para este artículo.

Artículos más leídos del mismo autor/a

1 2 3 4 > >>