Isolation of cecal probiotic bacteria and characterization in the M2 competitive exclusion medium
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Publicado:
dic. 1, 2012
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Resumen
For the isolation of lactic bacteria (LAB) and Bacillus spp. endospores from the cecum of broilers and their characterization in the M2
competitive exclusion medium, five adult and healthy H2 hybrid chickens were slaughtered at 45 days old. In the initial counting there was a high number of LAB (1012 CFU.g-1) with cecal mucus scrape and Bacillus (1010 CFU.g-1) endospores in their cecal contents. Inocula were obtained in MRS (Man, Rogosa and Sharpe) medium for LAB and nutrient medium for Bacillus spp. endospores. A dynamic microbial and pH counting was made at 0, 6, 12 h during incubation. The M2 medium was based on final molasses, yeasts and inorganic salts hydrolysate and inoculated with MRS and nutrient media, with incubation at 37?C for 24 h. Growth dynamics, pH, lactic acid production and reducing sugars at 0, 6, 18, 24 h were evaluated, as well as the growth speed and the duplication time. At 24 h of culture, there were lower pH (4.0) and reducing sugars (1.7 g.L-1) values and higher of lactic acid (16.0 g.L-1). The growth speed and the duplication time were less than one hour. There was high LAB and Bacillus spp. population. It is considered that the M2 competitive exclusion medium fulfills the characteristics for the growth of probiotic bacteria.
Key words: probiotic, competitive exclusion, lactic bacteria, bacillus, characterization
competitive exclusion medium, five adult and healthy H2 hybrid chickens were slaughtered at 45 days old. In the initial counting there was a high number of LAB (1012 CFU.g-1) with cecal mucus scrape and Bacillus (1010 CFU.g-1) endospores in their cecal contents. Inocula were obtained in MRS (Man, Rogosa and Sharpe) medium for LAB and nutrient medium for Bacillus spp. endospores. A dynamic microbial and pH counting was made at 0, 6, 12 h during incubation. The M2 medium was based on final molasses, yeasts and inorganic salts hydrolysate and inoculated with MRS and nutrient media, with incubation at 37?C for 24 h. Growth dynamics, pH, lactic acid production and reducing sugars at 0, 6, 18, 24 h were evaluated, as well as the growth speed and the duplication time. At 24 h of culture, there were lower pH (4.0) and reducing sugars (1.7 g.L-1) values and higher of lactic acid (16.0 g.L-1). The growth speed and the duplication time were less than one hour. There was high LAB and Bacillus spp. population. It is considered that the M2 competitive exclusion medium fulfills the characteristics for the growth of probiotic bacteria.
Key words: probiotic, competitive exclusion, lactic bacteria, bacillus, characterization
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Cómo citar
Laurencio, M., Rond?n, A., Mili?n, G., P?rez, M., Arteaga, F., & Bocourt, R. (2012). Isolation of cecal probiotic bacteria and characterization in the M2 competitive exclusion medium. Cuban Journal of Agricultural Science, 46(4). Recuperado a partir de https://www.cjascience.com/index.php/CJAS/article/view/155
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