In vitro metabolic activity of Lactobacillus salivarius and its effect on productive and health indicators of lactating calves

Abstract

In vitro metabolic activity of Lactobacillus salivarius C-65 (constituent of PROBIOLACTIL® bio-preparation) and its effect on productive and health indicators of lactating calves was evaluated. For this, carbohydrate fermentation and the production of specific enzymes were determined in vitro using API 50 CH and API 50 CHL, and API-ZYM galleries, respectively. Furthermore, growth capacity and fermentative activity of Lactobacillus salivarius were evaluated in RALTEC Milk 17-1 milk replacer for 10 hours. The probiotic effect of PROBIOLACTIL® was assessed on bio-productive and health indicators of lactating calves. As a result, this microorganism ferments sugars galactose, D-glucose, D-fructose, D-mannose, mannitol,
sorbitol, N-acetyl-glucosamine, maltose, lactose, melibiose, sucrose, trehalose, inulin and D-raffinose. It was demonstrated that it produces enzymes that intervene in cellulose degradation, and other specific enzymes. This bacterium grew (15 LN CFU.mL-1) in the milk replacer and decreased the pH (6.08-5.82) and total reducing sugars (44-29 mg. L-1) in 10 hours. Finally, it was observed that calves that consumed the two doses of PROBIOLACTIL® (10 and 20 mL) showed differences (P <0.05) when improving weight gain (8 vs. 16 kg), and daily mean gain (163 vs. 326 g), as well as reducing the incidence of diarrhea. It is concluded that the bio-prepared PROBIOLACTIL® presents high metabolic activity and is capable
of causing a probiotic effect on productive and health indicators of lactating calves.
Key words: probiotics, enzymes, health